Nutrition and Growth Kinetics in Nitrogen- or Phosphorus-limited Cultures of the Noxious Red Tide Dinoflagellate <i>Gymnodinium mikimotoi</i>

• Yamaguchi Mineo

  Harmful Phytoplankton Section, Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea

• Itakura Shigeru

  Harmful Phytoplankton Section, Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea

Bibliographic Information

Other Title

• Nutrition and Growth Kinetics in Nitrogen- or Phosphorus-limited Cultures of the Noxious Red Tide Dinoflagellate Gymnodinium mikimotoi

Abstract

The dinoflagellate, Gymnodinium mikimotoi, causes severe red tides which have been associated with mass mortality of marine fish and invertebrates in western Japan. To establish the background biological information to elucidate the mechanism of red tide outbreaks, nutrition and growth kinetics in nitrogen (N)- or phosphorus (P)-limited semi-continuous cultures were examined using an axenic strain. G. mikimotoi is able to grow using organic and inorganic N compounds as sole N sources. Nitrate, nitrite, and ammonium were found to be good nitrogen sources. Urea and uric acid were not so well utilized and none of the amino acids, except for glutamine and tryptophan, were utilized. G.mikimotoi was capable of using a wide variety of inorganic and organic phosphorus compounds of different molecular structure successfully as a sole P source. Under N-limited steady state conditions, dilution rate (=growth rate), as a function of cell nitrogen quota, followed the Droop equation. Similarly, dilution rate, as a function of cell P quota, followed the Droop equation under P-limited steady state culture. Kinetic parameters D_m and K_q obtained for N- and P-limited cultures were 0.54 day^-1 and 3.13 pmol-cell^-1, and 0.67 day^-1 and 0.25 pmol-cell^-1, respectively. The nutrient availability and kinetic parameters of G. mikimotoi were compared to other red tide organisms and the ecological implications of these characteristics were discussed.

Journal

• Fisheries science

  Fisheries science 65 (3), 367-373, 1999

  The Japanese Society of Fisheries Science

Keywords

• Gymnodinium mikimotoi
• dinoflagellate
• red tide
• nutrition
• semi-continuous culture
• nitrogen
• phosphorus
• minimum cell quota

Details

• CRID: 1390282679405552640
• NII Article ID: 130003903367
• NII Article ID: 10004878814
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.65.367
• COI: 1:CAS:528:DyaK1MXktlCmtro%3D
• NDL BIB ID: 4767334
• ISSN: 09199268
• ISSN: http://id.crossref.org/issn/09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4767334
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/65/3/65_3_367/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed