Phylogenetic Analysis of Algicidal Bacteria (Family Flavobacteriaceae) and Selective Detection by PCR Using a Specific Primer Set

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  • Kondo Ryuji
    Department of Marine Bioscience, Fukui Prefectural University
  • Imai Ichiro
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
  • Fukami Kimio
    Department of Aquaculture, Faculty of Agriculture, Kochi University
  • Minami Akiko
    Department of Marine Bioscience, Fukui Prefectural University
  • Hiroishi Shingo
    Department of Marine Bioscience, Fukui Prefectural University

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  • Phylogenetic Analysis of Algicidal Bacteria (Family <i>Flavobacteriaceae</i>) and Selective Detection by PCR Using a Specific Primer Set

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Sequencing the 16S rDNA and phylogenetic analysis were performed on algicidal bacteria belonging to the family Flavobacteriaceae, Chattonella-killing Cytophaga sp. strain J18/M01, Heterocapsa-killing Cytophaga sp. strains AA8-2 and AA8-3, and Gymnodinium growth-inhibiting Flavobacterium sp. strain 5N-3. These algicidal strains were clustered phylogenetically within the marine Cytophaga/Flavobacter/Bacteroides (CFB) group. Flavobacterium sp. 5N-3 was phylogenetically clustered with Psychroserpens burtonensis but not with the algicidal gliding bacteria. The sequence similarity between strain 5N-3 and P. burtonensis was only 92%. The strains J18/M01 and AA8-2 (AA8-3) exhibited the high level of sequence similarity of 98.5%. Despite the differences of host algae and of locality and year of isolation, these algicidal bacteria were phylogenetically located on the same terminal branch.<br> The PCR amplification of the 16S rDNA of the CFB strains using a single set of J18/M01-specifk primers resulted in specific detection of the J18/M01, indicating that the J18/M01-specific sequence in the 16S rDNA is useful for selective detection of the strain J18/M01.

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