Improvement of Emulsifying and Antibacterial Properties of Salmine by the Maillard Reaction with Dextran

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In order to improve the emulsifying and antibacterial properties of salmine (a protamine from salmon sperm), its conjugates with dextran (molecular weight 60, 000-90, 000) were prepared through the Maillard reaction above glass transition temperature (Tg). Development of brown color and formation of salmine-dextran conjugates were concomitantly accelerated at heating temperatures above Tg+30°C. The emulsifying activity of the conjugates became 4-5 times as high as that of salmine during the early stage of the Maillard reaction at 180-190°C, but decreased drastically at the advanced stage. The antibacterial activity of salmine was also increased by the conjugation with dextran. Even antibacterial activity against some species of Gram-negative bacteria was attained as a result of the conjugation. Potential use of salmine-dextran conjugates as bifunctional food additives is discussed.

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