Increased Levels of Mitochondrial ATP Synthase β-Subunit in Fast Skeletal Muscle of Carp Acclimated to Cold Temperature

  • Kikuchi Kiyoshi
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Itoi Shiro
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Watabe Shugo
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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タイトル別名
  • Increased Levels of Mitochondrial ATP Synthase β-Subunit in Fast Skeletal Muscle of Carp Acclimated to Cold Temperature
  • Increased Levels of Mitochondrial ATP Synthase ベーターSubunit in Fast Skeletal Muscle of Carp Acclimated to Cold Temperature

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We compared two-dimensional electrophoretic patterns of cytosolic extracts from fast muscle of the 10°C- and 30°C-acclimated carp Cyprinus carpio, and revealed that cold acclimation resulted in an approximately two-fold increase of the expression levels of a 55-kDa protein. This protein was identified as mitochondrial ATP synthase β-subunit by N-terminal amino acid sequencing after electrophoresis along with subsequent cDNA cloning. A full-length cDNA clone of carp ATP synthase β-subunit encoded 518 amino acids, showing 72-89% identity to those of eukaryotes at the level of amino acid sequence. The accumulated levels of ATP synthase β-subunit transcripts in the fast muscle from the 10βC- and 30βC-acclimated carp were well correlated with those at the protein level, suggesting that expression of this protein during temperature acclimation is regulated mainly at the transcription level.

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