Increased Levels of Mitochondrial ATP Synthase β-Subunit in Fast Skeletal Muscle of Carp Acclimated to Cold Temperature
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- Kikuchi Kiyoshi
- Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Itoi Shiro
- Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Watabe Shugo
- Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
書誌事項
- タイトル別名
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- Increased Levels of Mitochondrial ATP Synthase β-Subunit in Fast Skeletal Muscle of Carp Acclimated to Cold Temperature
- Increased Levels of Mitochondrial ATP Synthase ベーターSubunit in Fast Skeletal Muscle of Carp Acclimated to Cold Temperature
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We compared two-dimensional electrophoretic patterns of cytosolic extracts from fast muscle of the 10°C- and 30°C-acclimated carp Cyprinus carpio, and revealed that cold acclimation resulted in an approximately two-fold increase of the expression levels of a 55-kDa protein. This protein was identified as mitochondrial ATP synthase β-subunit by N-terminal amino acid sequencing after electrophoresis along with subsequent cDNA cloning. A full-length cDNA clone of carp ATP synthase β-subunit encoded 518 amino acids, showing 72-89% identity to those of eukaryotes at the level of amino acid sequence. The accumulated levels of ATP synthase β-subunit transcripts in the fast muscle from the 10βC- and 30βC-acclimated carp were well correlated with those at the protein level, suggesting that expression of this protein during temperature acclimation is regulated mainly at the transcription level.
収録刊行物
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- Fisheries science
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Fisheries science 65 (4), 629-636, 1999
公益社団法人 日本水産学会
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詳細情報
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- CRID
- 1390282679406151680
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- NII論文ID
- 130003742191
- 10004879950
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- NII書誌ID
- AA10993718
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- NDL書誌ID
- 4839854
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- ISSN
- 09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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