Extraction and Characterization of the Smooth-Type Lipopolysaccharide from Fusobacterium nucleatum JCM 8532 and Its Biological Activities
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- ONOUE Sakura
- Department of Preventive and Community Dentistry, The Nippon Dental University, School of Dentistry at Tokyo
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- NIWA Moto
- Department of Preventive and Community Dentistry, The Nippon Dental University, School of Dentistry at Tokyo
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- ISSHIKI Yasunori
- The Kitasato Institute
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- KAWAHAEA Kazuyoshi
- The Kitasato Institute
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Abstract
The lipopolysaccharide (LPS) of Fusobacterium nucleatum JCM 8532 was isolated by hot-phenol water extraction. Most of the LPS was extracted in the phenolic phase and shown to be the smooth-type, whereas the aqueous phase contained mainly rough-type LPS. The chemical composition of the LPS was similar to that reported in other studies, but D-quinovosamine, which may be a major component of O-antigenie polysaccharide, and 3-deoxy-D-manno-2-octulosonic acid (Kdo) were detected for the first time by gas chromatography-mass spectrometry. The biological activities of smooth-type LPS, including limulus activity, lethal toxicity, pyrogenicity, and B lymphocyte mitogenicity, were comparable to those of enterobacterial LPS. Smooth-type LPS inhibited the cell growth and DNA synthesis of adult and fetal human gingival fibroblasts in a dose-dependent manner, suggesting that LPS may play a role in the occurrence of human gingivitis.
Journal
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 40 (5), 323-331, 1996-05-20
Center For Academic Publications Japan
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Details
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- CRID
- 1571417124231627520
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- NII Article ID
- 10004892679
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- NII Book ID
- AA00738350
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- ISSN
- 03855600
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- Text Lang
- en
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- Data Source
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- CiNii Articles