Acid-Fastness of Nocardia asteroides GUH-2 Cultured in Brain Heart Infusion Broth Supplemented with Paraffin

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Abstract

Nocardia asteroides GUH-2 was not acid-fast when grown in brain heart infusion (BHI) broth. When grown in BHI broth supplemented with paraffin, many filamentous cells showed acid-fastness after treatment with 1% acid-alcohol as the decolorizing agent. When treated with 3% acid-alcohol, filamentous cells were not acid-fast. In addition to the acid-fast filamentous cells of nocardiae, unknown acidfast spherical bodies were observed in the paraffin-supplemented BHI broth cultures.

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