Characterization of Actinobacillus actinomycetemcomitans Hemolysin
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Twenty out of 33 <i>Actinobacillus actinomycetemcomitans</i> strains formed hemolytic colonies on horse blood agar plates under anaerobic conditions. The hemolytic activity found in <i>A. actinomycetemcomitans</i> strain 137HE was examined. This activity was detected in the late exponential to early stationary phases of growth. Human erythrocytes were the most susceptible, followed by rabbit, sheep, horse and swine red blood cells. The majority of activity was detected in the cell-associated vesicle fraction. Zwitterionic detergent 3-[(3-cholamidopropyl)-dimethyl-ammonio]-1-propanesulfonate (CHAPS) extract from whole cells was semipurified by ammonium sulfate precipitation, preparative isoelectric focusing (IEF) and gel-filtration chromatography to yield a major band on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with a molecular mass of 12kDa. Heating at 80C for 30min and treatment with proteinase K or trypsin resulted in complete disappearance of the hemolytic activity. Sulphydryl reagents enhanced activity and small amounts of cholesterol inhibited it. In summary, we demonstrated the presence of hemolysin in <i>A. actinomycetemcomitans</i>, and examined and characterized it.
- Japanese Journal of Microbiology
Japanese Journal of Microbiology 40(10), 717-723, 1996-10-20
Center For Academic Publications Japan