Detection and Identification of Yersinia pestis by Polymerase Chain Reaction (PCR) Using Multiplex Primers
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- TSUKANO Hiroko
- Department of Bacteriology, National Institute of Health
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- ITOH Ken-ichiro
- Department of Bacteriology, National Institute of Health
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- SUZUKI Sosuke
- Center for Infection of Imported Foods and Infectious Disease
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- WATANABE Haruo
- Department of Bacteriology, National Institute of Health
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Abstract
A PCR method for detection of Yersinia pestis-virulence determinants by the use of multiplex primers was developed. Four pairs of oligonucleotide primers were designed from each gene of three kinds of virulent plasmids and a chromosomal DNA; 60-Md plasmid-located gene (caf1) encoding Y. pestis-specific capsular antigen fraction 1, a Y. pestis-specific region of a yopM gene encoded on 42-Md virulent plasmid, a plasminogen activator gene (pla) encoded on Y. pestis-specific 7-Md plasmid and an invasin protein gene (inv) encoded on chromosomal DNA. This multiplex-primer system was specific for the detection of Y. pestis among pathogenic Yersinia species and other enterobacteriaceae having antigens common to Y. pestis. Since this method is simple and safe, it will be useful to identify and confirm Y. pestis in cases of emergency and for the surveillance of epidemics.
Journal
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 40 (10), 773-775, 1996-10-20
Center For Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1570009749348119040
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- NII Article ID
- 10004894532
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- NII Book ID
- AA00738350
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- ISSN
- 03855600
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- Text Lang
- en
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- Data Source
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- CiNii Articles