Detection and Identification of Yersinia pestis by Polymerase Chain Reaction (PCR) Using Multiplex Primers

• TSUKANO Hiroko

  Department of Bacteriology, National Institute of Health

• ITOH Ken-ichiro

  Department of Bacteriology, National Institute of Health

• SUZUKI Sosuke

  Center for Infection of Imported Foods and Infectious Disease

• WATANABE Haruo

  Department of Bacteriology, National Institute of Health

抄録

A PCR method for detection of Yersinia pestis-virulence determinants by the use of multiplex primers was developed. Four pairs of oligonucleotide primers were designed from each gene of three kinds of virulent plasmids and a chromosomal DNA; 60-Md plasmid-located gene (caf1) encoding Y. pestis-specific capsular antigen fraction 1, a Y. pestis-specific region of a yopM gene encoded on 42-Md virulent plasmid, a plasminogen activator gene (pla) encoded on Y. pestis-specific 7-Md plasmid and an invasin protein gene (inv) encoded on chromosomal DNA. This multiplex-primer system was specific for the detection of Y. pestis among pathogenic Yersinia species and other enterobacteriaceae having antigens common to Y. pestis. Since this method is simple and safe, it will be useful to identify and confirm Y. pestis in cases of emergency and for the surveillance of epidemics.

収録刊行物

• Microbiology and immunology

  Microbiology and immunology 40 (10), 773-775, 1996-10-20

  財団法人 学会誌刊行センター

キーワード

• PCR
• Yersinia pestis
• Multiplex primers
• Virulence genes

詳細情報

• CRID: 1570009749348119040
• NII論文ID: 10004894532
• NII書誌ID: AA00738350
• ISSN: 03855600
• Web Site: http://chemport.cas.org/cgi-bin/sdcgi?APP=ftslink&action=reflink&origin=jstage2&version=1.0&coi=1:CAS:528:DyaK2sXhtFSh&md5=3578b88f03079785352287167712b01e
• Web Site: https://search.jamas.or.jp/link/ui/1997042940
• 本文言語コード: en
• データソース種別:
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