Role of Cell Surface Glycosaminoglycans of Human T Cells in Human Immunodeficiency Virus Type-1 (HIV-1) Infection
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- OHSHIRO Yukako
- Departments of Microbiology, Tokyo Medical and Dental University School of Medicine
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- MURAKAMI Tsutomu
- Departments of Microbiology, Tokyo Medical and Dental University School of Medicine
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- MATSUDA Kazuhiro
- Departments of Microbiology, Tokyo Medical and Dental University School of Medicine
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- NISHIOKA Kiyoshi
- Departments of Dermatology, Tokyo Medical and Dental University School of Medicine
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- YOSHIDA Keiichi
- Tokyo Research Institute, Seikagaku Corporation
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- YAMAMOTO Naoki
- Departments of Microbiology, Tokyo Medical and Dental University School of Medicine
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To investigate the role of cell surface glycosaminoglycans (GAGs), including heparan sulfate (HS), on HIV-1 infection in human T cells, HIV-1 binding and infection were determined after treatment of T-cell lines and CD4+T cells from normal peripheral blood mononuclear cells (PBMC) with GAG-degrading enzyme or a GAG metabolic sulfation inhibitor. Heparitinase I (hep I) and sodium chlorate prevented binding of HIV-1/IIIB to MT-4 cells as revealed by indirect immunofluorescence procedures, thereby inhibiting infection. Hep I was less effective in the binding inhibition of the macrophage-tropic strain HIV-1/SF162 than that of the T-cell line-tropic strain HIV-1/IIIB. The binding of HIV-1/SF162 was about 100-fold less dependent on cell surface HS than HIV-1/IIIB. Human HTLV-I positive T-cell lines expressed more HS than HTLV-I negative T-cell lines or normal CD4+ T cells when stained with anti-HS mAbs against either native or heparitinase-treated HS. With the exception of endo-β-galactosidase (endo-β-gal), GAG-degrading enzymes, including hep I, chondroitinase ABC (chon ABC), chondroitinase AC II (chon AC II) and keratanase, did not prevent the binding of HIV-1/IIIB to CD4+ T cells from normal PBMC. These results indicate that the cell surface HS of human T cells participates in HIV-1 infection by facilitating HIV-1/IIIB binding to MT-4 cells. In particular, the sulfation of HS chains is critical. Since the expression of cell surface HS varies among T cells, which are not consistently sensitive to hep I treatment in HIV-1 binding inhibition, other GAG-like molecules may also be involved.
収録刊行物
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- Microbiology and immunology
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Microbiology and immunology 40 (11), 827-835, 1996-11-20
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詳細情報 詳細情報について
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- CRID
- 1572261549161666688
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- NII論文ID
- 10004894702
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- NII書誌ID
- AA00738350
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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