Differential Expression of a Mycobacterium tuberculosis Protein Recognized by a Mouse Monoclonal Antibody
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- CUMMINGS Patrick J.
- Department of Medical and Research Technology, University of Maryland School of Medicine
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- ROWLAND Sharon S.
- Department of Medical and Research Technology, University of Maryland School of Medicine
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- HOOPER Nancy E.
- Department of Medical and Research Technology, University of Maryland School of Medicine
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- SCHWALBE Richard S.
- Department of Medical and Research Technology, University of Maryland School of Medicine
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Abstract
Murine monoclonal antibodies were produced against Mycobacterium tuberculosis (Mtb) using standard hybridoma procedures. By a whole cell enzyme-linked immunosorbent assay (ELISA), one monoclonal antibody (mAb), HB28, demonstrated high level specific reactivity to Mtb. Western blot analysis demonstrated reactivity to a single 65kDa Mtb protein in the cell wall extract and culture filtrate. HB28 mAb appears to be recognizing a 65kDa Mtb protein that is over-expressed by Mtb but not other species under certain culture conditions. Differential expression and detection of this protein by HB28 mAb may have potential for diagnostic applications.
Journal
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 40 (11), 883-886, 1996-11-20
Center For Academic Publications Japan
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Details
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- CRID
- 1572543024138439808
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- NII Article ID
- 10004894917
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- NII Book ID
- AA00738350
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- ISSN
- 03855600
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- Text Lang
- en
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- Data Source
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- CiNii Articles