Differential Expression of a Mycobacterium tuberculosis Protein Recognized by a Mouse Monoclonal Antibody

  • CUMMINGS Patrick J.
    Department of Medical and Research Technology, University of Maryland School of Medicine
  • ROWLAND Sharon S.
    Department of Medical and Research Technology, University of Maryland School of Medicine
  • HOOPER Nancy E.
    Department of Medical and Research Technology, University of Maryland School of Medicine
  • SCHWALBE Richard S.
    Department of Medical and Research Technology, University of Maryland School of Medicine

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Abstract

Murine monoclonal antibodies were produced against Mycobacterium tuberculosis (Mtb) using standard hybridoma procedures. By a whole cell enzyme-linked immunosorbent assay (ELISA), one monoclonal antibody (mAb), HB28, demonstrated high level specific reactivity to Mtb. Western blot analysis demonstrated reactivity to a single 65kDa Mtb protein in the cell wall extract and culture filtrate. HB28 mAb appears to be recognizing a 65kDa Mtb protein that is over-expressed by Mtb but not other species under certain culture conditions. Differential expression and detection of this protein by HB28 mAb may have potential for diagnostic applications.

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