Optical Cofiguration Analysis of Hydorxy Fatty Acids in Bacterial Lipids by Chiral Colunm Hygh-Performance Liquid Chromatography
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Through the adoption of a chiral stationary phase in high-performance liquid chromatography and a simple derivatization method for hydroxy fatty acids, it became easy to separate and identify the optical isomers of 2- and 3-hydroxy fatty acids composing several kinds of microbial lipids. The 2- and 3-hydroxy fatty acids were converted with dinitrophenyl isocyanate to their 3, 5-dinitrophenyl urethane derivatives (DU-derivatives), which were analyzable by HPLC using a chiral column. By varying the composition of an eluent, separation of the DU-derivatives of hydroxy fatty acids differing in optical configuration, chain length and position of hydroxyl group was achieved. The general elution orders of these DU-derivatives were determined with authentic 2- and 3-hydroxy fatty acids. Small amounts (-300μg) of ornithine-containing lipids isolated from the <i>Serratia marcescens</i> strains were examined by this method to identify 3-hydroxy fatty acids of the lipids as D isomers.
- Japanese Journal of Microbiology
Japanese Journal of Microbiology 41(1), 27-32, 1997-01-20
Center For Academic Publications Japan