Unification of the Genera Serpulina and Brachyspira, and Proposals of Brachyspira hyodysenteriae Comb.Nov., Brachyspira innocens Comb.Nov.and Brachyspira pilosicoli Comb.Nov.

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The phylogenetic positions of <i>Serpulina hyodysenteriae, Serpulina innocens, Serpulina pilosicoli</i> and <i>Brachyspira aalborgi</i> were studied. Complete 16S ribosomal DNA sequences of these three species and <i>B. aalborgi</i> revealed that their 16S rDNA sequences were related more than 96.0%. The mol% guanine plus cytosine (G+C) of <i>B. aalborgi</i> DNA was 27.1, and was similar to those of the 3 members of the genus <i>Serpulina</i>. The homologous rates using <sup>32</sup>P-labeled <i>B. aalborgi</i> chromosome DNA in DNA-DNA reassociation tests were 22.0% to <i>S. hyodysenteriae</i>, 19.1% to <i>S. innocens</i> and 17.2% to <i>S. pilosicoli</i>. Therefore, we propose to transfer the three species of the genus <i>Serpulina</i> to the genus <i>Brachyspira</i>. Descriptions of <i>Brachyspira hyodysenteriae</i> comb. nov., <i>Brachyspira innocens</i> comb. nov. and <i>Brachyspira pilosicoli</i> comb. nov., and an emended description of <i>B. aalborgi</i> are given. Phenotypic characteristics of the 4 members of the genus <i>Brachyspira</i> were also studied. They fermented fructose, galactose, glucose, lactose, maltose, mannose, raffinose and trehalose; however, <i>B. aalborgi</i> did not ferment raffinose. All of them hydrolyzed esculin but did not produce indole except for <i>B. hyodysenteriae</i>. The protein profile of <i>B. aalborgi</i> was different from those of the four strains of <i>B. hyodysenteriae, B. innocens</i> and <i>B. pilosicoli</i>, but the heavy bands with molecular sizes of 49.4 and 52.3kDa of <i>B. aalborgi</i> were quite similar to those of <i>B. innocens</i> in the points of quantity and molecular size. In immunoblotting tests, <i>B. aalborgi</i> reacted well with anti-<i>B. innocens</i> and <i>B. pilosicoli</i> sera, but reacted weakly with anti-<i>B. hyodysenteriae</i> serum. Only one heavy band and several faint bands were revealed by the reaction between <i>B. aalborgi</i> and anti-<i>B. hyodysenteriae</i> serum, and the heavy band was common among these strains.


  • Japanese Journal of Microbiology  

    Japanese Journal of Microbiology 41(6), 445-452, 1997-06-20 

    Center For Academic Publications Japan

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