Intestinal Distribution and Intraluminal Localization of Orally Administered Clostridium butyricum in Rats
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<i>Clostridium butyricum</i> has been used as a probiotic in animals and humans for years, however, its fate in the intestine has not been clarified yet. We investigated the intestinal fate of <i>C. butyricum</i> using a selective medium and a monoclonal antibody after orally administering <i>C. butyricum</i> spores to rats. The number of <i>C. butyricum</i>, both viable and dead cells, in the intestinal contents were counted by enzyme-linked immunosorbent assay (ELISA) at various times after a single oral administration. The total viable number of <i>C. butyricum</i> was counted using a selective medium, and viable resting spores were selectively detected by treating the samples with ethanol. To investigate the intraluminal localization of the <i>C. butyricum</i> cells, frozen intestinal tracts were imprinted onto slides and stained with immunogold-silver. Total viable spores exceeded the number of viable resting spores by more than 10-fold from the proximal to middle of the small intestine 30min after administration. Vegetative cells of <i>C. butyricum</i> were first detected in the distal small intestine after 2hr, and vegetative growth was observed from the cecum to the colon 5hr after administration. Dead vegetative cells were detected 9hr after administration, and <i>C. butyricum</i> cells were not detected in the intestine after 3 days. The <i>C. butyricum</i> cells in the intestinal imprints were stained specifically by immunogold-silver staining, and proliferative cells were observed in the cecum after 3hr. These results suggest that the administered <i>C. butyricum</i> germinated in the upper small intestine, grew mainly from the distal small intestine to the colon and were excreted from the rat intestine within 3 days.
- Japanese Journal of Microbiology
Japanese Journal of Microbiology 41(9), 665-671, 1997-09-20
Center For Academic Publications Japan