Intestinal Distribution and Intraluminal Localization of Orally Administered Clostridium butyricum in Rats

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<i>Clostridium butyricum</i> has been used as a probiotic in animals and humans for years, however, its fate in the intestine has not been clarified yet. We investigated the intestinal fate of <i>C. butyricum</i> using a selective medium and a monoclonal antibody after orally administering <i>C. butyricum</i> spores to rats. The number of <i>C. butyricum</i>, both viable and dead cells, in the intestinal contents were counted by enzyme-linked immunosorbent assay (ELISA) at various times after a single oral administration. The total viable number of <i>C. butyricum</i> was counted using a selective medium, and viable resting spores were selectively detected by treating the samples with ethanol. To investigate the intraluminal localization of the <i>C. butyricum</i> cells, frozen intestinal tracts were imprinted onto slides and stained with immunogold-silver. Total viable spores exceeded the number of viable resting spores by more than 10-fold from the proximal to middle of the small intestine 30min after administration. Vegetative cells of <i>C. butyricum</i> were first detected in the distal small intestine after 2hr, and vegetative growth was observed from the cecum to the colon 5hr after administration. Dead vegetative cells were detected 9hr after administration, and <i>C. butyricum</i> cells were not detected in the intestine after 3 days. The <i>C. butyricum</i> cells in the intestinal imprints were stained specifically by immunogold-silver staining, and proliferative cells were observed in the cecum after 3hr. These results suggest that the administered <i>C. butyricum</i> germinated in the upper small intestine, grew mainly from the distal small intestine to the colon and were excreted from the rat intestine within 3 days.

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  • Microbiology and immunology  

    Microbiology and immunology 41(9), 665-671, 1997-09-20 

    Center For Academic Publications Japan

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各種コード

  • NII論文ID(NAID)
    10004897805
  • NII書誌ID(NCID)
    AA00738350
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    03855600
  • NDL 記事登録ID
    4317093
  • NDL 雑誌分類
    ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
  • NDL 請求記号
    Z53-M387
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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