Intestinal Distribution and Intraluminal Localization of Orally Administered Clostridium butyricum in Rats

• Sato Rumiko

  Miyarisan Pharmaceutical Co.

• Tanaka Mamoru

  Miyarisan Pharmaceutical Co.

書誌事項

タイトル別名

• Intestinal Distribution and Intraluminal Localization of Orally Administered <i>Clostridium butyricum</i> in Rats
• Intestinal Distribution and Intralumina

抄録

Clostridium butyricum has been used as a probiotic in animals and humans for years, however, its fate in the intestine has not been clarified yet. We investigated the intestinal fate of C. butyricum using a selective medium and a monoclonal antibody after orally administering C. butyricum spores to rats. The number of C. butyricum, both viable and dead cells, in the intestinal contents were counted by enzyme-linked immunosorbent assay (ELISA) at various times after a single oral administration. The total viable number of C. butyricum was counted using a selective medium, and viable resting spores were selectively detected by treating the samples with ethanol. To investigate the intraluminal localization of the C. butyricum cells, frozen intestinal tracts were imprinted onto slides and stained with immunogold-silver. Total viable spores exceeded the number of viable resting spores by more than 10-fold from the proximal to middle of the small intestine 30min after administration. Vegetative cells of C. butyricum were first detected in the distal small intestine after 2hr, and vegetative growth was observed from the cecum to the colon 5hr after administration. Dead vegetative cells were detected 9hr after administration, and C. butyricum cells were not detected in the intestine after 3 days. The C. butyricum cells in the intestinal imprints were stained specifically by immunogold-silver staining, and proliferative cells were observed in the cecum after 3hr. These results suggest that the administered C. butyricum germinated in the upper small intestine, grew mainly from the distal small intestine to the colon and were excreted from the rat intestine within 3 days.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 41 (9), 665-671, 1997

  財団法人 学会誌刊行センター

キーワード

• Virology
• Immunology
• Microbiology

詳細情報

• CRID: 1390282681299645312
• NII論文ID: 10004897805
• NII書誌ID: AA00738350
• DOI: 10.1111/j.1348-0421.1997.tb01909.x
• COI: 1:CAS:528:DyaK2sXmslyntr4%3D
• ISSN: 13480421
• ISSN: 03855600
• ISSN: http://id.crossref.org/issn/03855600
• NDL書誌ID: 4317093
• PubMed: 9343816
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4317093
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1348-0421.1997.tb01909.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1348-0421.1997.tb01909.x
• Web Site: https://search.jamas.or.jp/link/ui/1998086541
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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