Fluorometric Determination of Glucose by Flow Injection Analysis Using Immobilized Enzyme-Reactor Columns Prepared by Coupling Glucose Oxidase and Peroxidase onto Chitosan Beads

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抄録

A combination technique of some kinds of enzymatic reactions for flow injection analysis with an immobilized enzyme-reactorcolumn was studied. This system consisted of hand-made reactor columns packed with glucose oxidase and horseradish peroxidase immobilized onto chitosan beads, and a fluorometric detector. It was applied for the determination of glucose in some beverages, Japanese Sake and liquor samples. Under the recommended conditions, it could determinea sample within 10 min. The calibration curve for glucose was linear from 20.0 mg dm-3 to 0.5 mg dm-3 in the low-sensitivityregion of the detector, and from 0.5 mg dm-3 to 30 g dm-3 in the high-sensitivity region of the detector. The detection limit (S/N=10) was 30 µg dm-3 as the concentration of glucose, and 54 ng as its absolute amount. There was no interference from major substances present in either beverage or serum samples.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry  

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 16(5), 517-521, 2000-05-10 

    社団法人 日本分析化学会

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各種コード

  • NII論文ID(NAID)
    10004954708
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    5384563
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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