Application of Horseradish Peroxidase-Encapsulated Liposomes as Labels for Immunodotblotting.

DOI HANDLE PDF Web Site Web Site View 1 Remaining Hide 5 Citations 14 References Open Access

Search this article

Abstract

Liposomes are spherical vesicles consisting of phospholipid bilayers surrounding an aqueous volume. Recently, attention has focused on liposomes as a signal-enhancement agent, since a number of marker molecules can be encapsulated in their aqueous interior. An antibody labeled with liposome encapsulating enzyme, such as glucose oxidase, has been employed for immunoassays. On the other hand, HRP-conjugated liposomes were prepared by covalently attaching HRP to the outside of the lipid bilayer of liposome. However, the preparation of HRP-conjugated liposomes was tedious and time-consuming, since several reaction steps are required for linking HRP covalently to the surface of liposomes. In order to hold simply HRP in liposomes, we examined how to encapsulate HRP into the aqueous interior of liposomes prepared by an extrusion technique (VETs).4 In addition, the HRP-encapsulated VETs were coupled covalently to anti-rabbit IgG(antibodytagged VETs).5 the detection of HRP encapsulated in the antibody-tagged VETs was made by luminol chemiluminescent (CL) method. On the other hand, biotin-tagged molecules are currently more universal as a marker in immunoassays compared to antibody-tagged marker moleucles. In the present study, the HRP-encapsulated liposomes containing biotinylated dipalmitoylphosphatidylethanolamin were prepared by an extrusion technique (biotin-tagged VETs). In addition, biotin-tagged VETs were applied to labels in immunodotblotting of rabbit IgG.

Journal

  • Analytical Sciences

    Analytical Sciences 16 (5), 527-529, 2000

    The Japan Society for Analytical Chemistry

Citations (5)*help

See more

References(14)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top