Rapid Determination of Benzene Metabolites Phenol and p-Cresol in the Urine of Petrol Station Workers by Gas Chromatography
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The determination of phenol and p-cresol in urine has been suggested as a suitable biomarker for the monitoring of benzene exposure of pump workers employed in petrol stations. A method for efficiently extracting a wide variety of drugs from urine for toxicological analysis by gas chromatography is presented. The sensitivity of the method is sufficient to monitor normal levels of phenol and p-cresol after exposure to benzene vapors. The detection limit of the method is low enough to measure urinary phenol and p-cresol at a concentration of 0.1 mg/l. The overall recoveries of urinary phenol and p-cresol relative to the internal standard, DPA, ranged between 95 and 96.5%. Our gas chromatographic method is a rapid, precise and economical one suitable to measure the metabolite benzene also in routine monitoring of phenol and p-cresol.
- Analytical Sciences
Analytical Sciences 16(6), 589-591, 2000-06-10
The Japan Society for Analytical Chemistry