Amperometric Biosensors for Sensing of Hydrogen Peroxide Based on Electron Transfer between Horseradish Peroxidase and Ferrocene as a Mediator

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A non-conducting polymer film of 3-aminophenol, in which horseradish peroxidase was immobilized, has been prepared on a carbon paste electrode containing both ferrocene as a mediator and horseradish peroxidase by the electrochemical polymerization. The response currents of this hydrogen peroxide sensor linearly increased with increasing the concentration of hydrogen peroxide of 0.6 to 20 μmol dm-3; the straight line had a slope equal to 10.1 mA mol-1 dm3 (r= 0.999). This hydrogen peroxide sensor may be available for the fabrication of other biosensors, e.g. glucose and cholesterol sensors, which are not influenced by easily oxidizable species, <font size=-2>L</font>-ascorbic acid and uric acid.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry  

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 16(6), 609-613, 2000-06-10 

    社団法人 日本分析化学会

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各種コード

  • NII論文ID(NAID)
    10004955098
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    5380264
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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