A novel minisatellite repeat expansion identified at FRA16B in a Japanese carrier.
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- Yamauchi Masatake
- Genome Research Group, National Institute of Radiological Sciences
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- Tsuji Satsuki
- Genome Research Group, National Institute of Radiological Sciences
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- Mita Kazuei
- Genome Research Group, National Institute of Radiological Sciences
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- Saito Toshiyuki
- Genome Research Group, National Institute of Radiological Sciences
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- Morimyo Mitsuoki
- Genome Research Group, National Institute of Radiological Sciences
書誌事項
- タイトル別名
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- novel minisatellite repeat expansion identified at FRA16B in a Japanese carrier
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抄録
Previously, the allelic expansion of a 33-bp AT-rich minisatellite repeat has been reported to cause FRA16B, a distamycin A-inducible fragile site. Here, we identified a novel 35-bp minisatellite repeat at FRA16B in a Japanese carrier. The nucleotide sequence of the 35-bp minisatellite was highly AT-rich and nearly identical to the 33-bp one but with insertion of two nucleotides, thymine and adenine. The copy number of the AT-rich minisatellite was 21 in total in the carrier, while only a few copies of the 33-bp minisatellite were present in a non-carrier Japanese subject. These results suggest that the molecular mechanism involved in the allelic expansion of the minisatellite repeat in FRA16B recognizes both minisatellites, the 33-bp one and the 35-bp one, as an amplicon. These observations were different from the ones at folate-sensitive fragile sites, where the CCG triplet repeat was commonly involved in the allelic expansion. Although a slight reduction in AT content (95%>90%) in the region of minisatellite expansion in the carrier subject was observed, both AT-content and length of the highly AT-rich region seem to play important roles in the cytogenetic expression of the distamycin A-inducible fragile site. In another normal subject, without fragile site expression, allelic expansion involving the 33-bp minisatellite was observed, and the length of the AT-rich DNA region was increased up to ~1000 bp. Since the length of the AT-rich minisatellite region was increased up to ~1,100-bp in the carrier subject, the threshold length for the cytogenetic expression of the AT-rich DNA region may be between about 1,000-bp and 1,100-bp.<br>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 75 (3), 149-154, 2000
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390282680421958016
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- NII論文ID
- 10004957446
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- NII書誌ID
- AA11077421
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- COI
- 1:CAS:528:DC%2BD3cXms1ylurw%3D
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 5475568
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- PubMed
- 10984839
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可