A linkage map of the turnip sawfly Athalia rosae (Hymenoptera:Symphyta) based on random amplified polymorphic DNAs

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A linkage map was constructed for the sawfly, <i>Athalia rosae</i> (Hymenoptera), based on the segregation of random amplified polymorphic DNA (RAPD) markers and a visible mutation, <i>yellow fat body</i> (<i>yfb</i>). Forty haploid male progeny (20 <i>yfb</i> and 20 +) from a single diploid female parent (<i>yfb</i>/+) were examined. Sixty-one of the 180 arbitrary primers tested by polymerase chain reaction (PCR) produced one or more RAPD bands. A total of 79 RAPD markers were detected. Of these, seven showed significant deviation from the expected 1:1 ratio, and were therefore excluded from further analysis. The remaining 72 RAPD markers and the marker mutation, <i>yfb</i>, were subjected to linkage analysis. Sixty RAPD markers and the <i>yfb</i> marker were organized into 16 linkage groups, spanning a distance of 517.2 cM. Twelve RAPD markers showed no linkage relationship to any group. Thirteen gel-purified RAPD bands were cloned and sequenced to generate the sequence-tagged sites (STSs). A single locus was represented by two markers, with one of them having a short internal deletion.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 75(3), 159-166, 2000

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10004957492
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    5475578
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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