A linkage map of the turnip sawfly Athalia rosae (Hymenoptera: Symphyta) based on random amplified polymorphic DNAs.
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- Nishimori Yoshikatsu
- Graduate School of Science and Technology, Kobe University
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- Lee Jae Min
- Graduate School of Science and Technology, Kobe University
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- Sumitani Megumi
- Graduate School of Science and Technology, Kobe University
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- Hatakeyama Masatsugu
- Department of Biology, Faculty of Science, Kobe University
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- Oishi Kugao
- Graduate School of Science and Technology, Kobe University Department of Biology, Faculty of Science, Kobe University
Bibliographic Information
- Other Title
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- linkage map of the turnip sawfly Athalia rosae Hymenoptera Symphyta based on random amplified polymorphic DNAs
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Abstract
A linkage map was constructed for the sawfly, Athalia rosae (Hymenoptera), based on the segregation of random amplified polymorphic DNA (RAPD) markers and a visible mutation, yellow fat body (yfb). Forty haploid male progeny (20 yfb and 20 +) from a single diploid female parent (yfb/+) were examined. Sixty-one of the 180 arbitrary primers tested by polymerase chain reaction (PCR) produced one or more RAPD bands. A total of 79 RAPD markers were detected. Of these, seven showed significant deviation from the expected 1:1 ratio, and were therefore excluded from further analysis. The remaining 72 RAPD markers and the marker mutation, yfb, were subjected to linkage analysis. Sixty RAPD markers and the yfb marker were organized into 16 linkage groups, spanning a distance of 517.2 cM. Twelve RAPD markers showed no linkage relationship to any group. Thirteen gel-purified RAPD bands were cloned and sequenced to generate the sequence-tagged sites (STSs). A single locus was represented by two markers, with one of them having a short internal deletion.<br>
Journal
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- Genes & Genetic Systems
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Genes & Genetic Systems 75 (3), 159-166, 2000
The Genetics Society of Japan
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Details 詳細情報について
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- CRID
- 1390001205445249920
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- NII Article ID
- 10004957492
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- NII Book ID
- AA11077421
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- COI
- 1:CAS:528:DC%2BD3cXms1ylur0%3D
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- ISSN
- 18805779
- 13417568
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- NDL BIB ID
- 5475578
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- PubMed
- 10984841
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed