Ｃｕｔ‐Ｏｐｅｎ法　　Ｘｅｎｏｐｕｓ卵母細胞で安定した膜電流の測定と細胞内潅流を同時に可能にする新しい手法

DOI Web Site PubMed 参考文献15件

書誌事項

タイトル別名
  • 'Cut-Open' method: A new method for Xenopus oocytes that enables intracellular perfusion and stable current recording.
  • Cut Openホウ Xenopus ランボ サイボウ デ アンテイシタ マク

この論文をさがす

抄録

Xenopus oocytes are one of the most widely utilized expression systems for voltage-dependent ion channels. Here, I describe a new technique for voltage-clamp recording of Xenopus oocytes that enables fast and stable monitoring of transmembrane current as well as intracellular perfusion by cutting a part of the plasma membrane to open the cytoplasm to the recording chamber which is filled with artificial intracellular solution. The original method was developed and described by Dr. E. Stefani and his colleagues, and I have modified it by employing a push-pull cannula to exchange the intracellular solution. The main features of this technique are: 1) High frequency response and relatively low current noise; fast activation and deactivation of ionic currents can be precisely evaluated. 2) Stable recording conditions lasting for several hours; this is a marked advantage over the conventional and patch-clamp recording. 3) Control of the ionic composition of both the internal and external media; the cut-open configuration enables desired manipulation of the internal milieu of oocytes easily, which is suitable for the study of Ca2+ channel modulation by second messengers and drugs.

収録刊行物

  • 日本薬理学雑誌

    日本薬理学雑誌 111 (3), 157-166, 1998

    公益社団法人 日本薬理学会

参考文献 (15)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ