Induction of Heat Shock Protein 70 and Nucleolin and Their Intracellular Distribution during Early Stage of Liver Regeneration

• KONISHI Tetsumi

  Department of Biochemistry, School of Medicine, University of Occupational and Environmental Health

• KARASAKI Yuji

  Department of Biochemistry, School of Medicine, University of Occupational and Environmental Health

• NOMOTO Minoru

  Department of Molecular Biology School of Medicine, University of Occupational and Environmental Health

• OHMORI Hisamitsu

  Department of Biochemistry, School of Medicine, University of Occupational and Environmental Health

• SHIBATA Kiyotaka

  Department of Biotechnology, Ishinomaki-Senshu University

• ABE Tetsuya

  Department of Biochemistry, School of Medicine, University of Occupational and Environmental Health

• SHIMIZU Kenji

  Department of Biochemistry, School of Medicine, University of Occupational and Environmental Health

• ITOH Hideaki

  Department of Surgery I, School of Medicine, University of Occupational and Environmental Health

• HIGASHI Ken

  Department of Biochemistry, School of Medicine, University of Occupational and Environmental Health

抄録

Our previous work demonstrated a simultaneous induction of Hsp70 and nucleolin mRNA during the prereplicative stage of hepatocytes after partial hepatectomy [Ohmori, H. et al. (1990) Exp. Cell Res. 189, 227-232]. In the present study, changes of intracellular localization of these proteins were examined. Nucleolin, which mainly localized in the nucleus, increased with time and accumulated in the nucleolus around 12-18h after partial hepatectomy. Hsp70 protein also increased slightly around 6-12h after the operation. In accordance with this increase, immunohistochemical staining revealed that almost all nuclei of hepatocytes became Hsp70-positive, although Hsp70 was seen to be dispersed throughout the nucleoplasm and nucleolus at all times examined. Next, we isolated a cDNA clone of ribosomal protein (S-17) and examined its behavior. Induction of S-17 mRNA was observed to be essentially similar to that of nucleolin mRNA in regenerating rat liver, although inductions of histone H2A and H4 occurred at a later time, that is, in parallel with DNA synthesis. Furthermore, we observed a simultaneous induction of Hsp70 and nucleolin mRNA by serum-stimulation after serum-depletion in HeLa and IAR-20 (rat) culture cells. These results suggest that the induction of Hsp70, in addition to nucleolin, was not fortuitous but may be involved in the early events of liver regeneration.

収録刊行物

• The journal of biochemistry

  The journal of biochemistry 117 (6), 1170-1177, 1995-06-01

  社団法人 日本生化学会

キーワード

• G_1 phase
• Hsp 70
• nuclear localization
• nucleolin
• regenerating liver (rat)

詳細情報

• CRID: 1570854174378022528
• NII論文ID: 10005181535
• NII書誌ID: AA00694073
• ISSN: 0021924X
• Web Site: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=7490256&dopt=AbstractPlus
• Web Site: https://search.jamas.or.jp/link/ui/1996004974
• 本文言語コード: en
• データソース種別:
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