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- KATO Takashi
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- OGAMI Kinya
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- SHIMADA Yoshihiro
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- IWAMATSU Akihiro
- Central Laboratories for Key Technology, Kirin Brewery Co., Ltd.
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- SOHMA Yoshiaki
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- AKAHORI Hiromichi
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- HORIE Kaori
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- KOKUBO Atsuko
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- KUDO Yoko
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- MAEDA Emiko
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- KOBAYASHI Keiko
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- OHASHI Hideya
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- OZAWA Tadashi
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- INOUE Hideo
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- KAWAMURA Kazuo
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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- MIYAZAKI Hiroshi
- Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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抄録
A thrombopoietic factor, termed thrombopoietin (TPO), was highly purified directly from the plasma of sublethally irradiated 1, 100 rats by measuring the production of megakaryocytes from a highly enriched population of rat megakaryocyte progenitor cells (CFU-MK). The rat plasma TPO is a glycoprotein and strongly hydrophobic. The total activity and purification yields obtained were about 29% and 1.49×108, respectively. The amino acid sequences of the two peptide fragments prepared from the purified 19 kDa TPO were analyzed, and used for the cloning of rat and human TPO eDNAs. It was found that the 19 kDa TPO was truncated but comprised at least 163 amino acids. The sequence of human TPO eDNA revealed that the TPO was identical to the c-Mpl ligand. Both rat and human TPOs expressed in COS-1 cells exhibited significant activity toward the CFU-MK in vitro, and were active in stimulating platelet production in mice. These results indicate that a thrombopoietic factor originally found in the irradiated rat plasma is a ligand for the rat c-Mpl.
収録刊行物
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- The journal of biochemistry
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The journal of biochemistry 118 (1), 229-236, 1995-07-01
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1573387449168444928
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- NII論文ID
- 10005183380
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- NII書誌ID
- AA00694073
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- ISSN
- 0021924X
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles