NMR of All-Carbon-13 Sugars: An Application in Development of an Analytical Method for a Novel Natural Sugar, 1,5-Anhydrofructose

Web Site 被引用文献3件 参考文献9件
  • KAMETANI Shunichi
    Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo
  • MIZUNO Hideaki
    Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo
  • SHIGA Yoko
    Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo
  • AKANUMA Hiroshi
    Department of Life Science (Chemistry), Graduate School of Arts and Sciences, The University of Tokyo

この論文をさがす

抄録

Of the all-carbon-13 compounds, glucose is one of the most easily accessible, and therefore we applied 13C-NMR technique to the metabolic study of glucose-related compounds, 1, 5-anhydro-D-glucitol and 1, 5-anhydro-D-fructose (AF). Applying an INADEQUATE method to the substitutes of these novel sugars fully labeled with carbon-13, we could trace out the entire carbon skeleton with high sensitivity and confirm the chemical structures of these sugars. The method also provided a much easier way to optimize the enzymatic oxidation for AF preparation: we selectively and continuously monitored the quantities, as well as their structures in aqueous solution, of the substrate and products in a noninvasive manner. Similarly relying upon information from the 13C-NMR, we developed a valuable derivatization method of AF for its GC-MS application, which was so sensitive that we were able to demonstrate the natural occurrence of AF in rat liver.

収録刊行物

被引用文献 (3)*注記

もっと見る

参考文献 (9)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ