Construction and Characterization of Glucose Enzyme Sensor Employing Engineered Water Soluble PQQ Glucose Dehydrogenase with Improved Thermal Stability
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- TAKAHASHI Yuka
- Department of Biotechnology, Tokyo University of Agriculture and Technology
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- IGARASHI Satoshi
- Department of Biotechnology, Tokyo University of Agriculture and Technology
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- NAKAZAWA Yukie
- Department of Biotechnology, Tokyo University of Agriculture and Technology
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- TSUGAWA Wakako
- Department of Biotechnology, Tokyo University of Agriculture and Technology
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- SODE Koji
- Department of Biotechnology, Tokyo University of Agriculture and Technology
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<p>In this study, we constructed glucose enzyme sensors with increased thermal stability employing engineered PQQGDH-B. First, we demonstrated the enzyme glucose sensor employing an engineered PQQGDH-B, Ser231 Lys. The residual activity after heat treatment at 60°C for 2 hours of the enzyme electrode employed Ser231Lys was 80% of the initial activity, whereas the electrode employed native PQQGDH-B was 30%. Second, we investigated the effect of cross-linking chemical modification on the thermal stability of PQQGDH-B. The cross-linked PQQGDH-B had high thermal stability and the half life time at 55°C was 63 min whereas that of native enzyme was 4 min. Furthermore, we demonstrated the Flow Injection Analysis (FIA) system for glucose measurement employed the cross-linked PQQGDH-B. The high operational stability of this system showed the very stable responses (SD was within 3%) for 100 times of glucose injection.</p>
収録刊行物
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- Electrochemistry
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Electrochemistry 68 (11), 907-911, 2000-11-05
公益社団法人 電気化学会
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詳細情報 詳細情報について
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- CRID
- 1390001277391560192
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- NII論文ID
- 10005360136
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- NII書誌ID
- AN00151637
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- ISSN
- 21862451
- 13443542
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- NDL書誌ID
- 5541716
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用可