四塩化炭素急性肝障害ラットにおけるアルブミン遺伝子発現の調節機構

• 小原 啓彦

  岩手医科大学内科学第一

• 桑波田 雅士

  徳島大学医学部栄養化学

• 加藤 章信

  岩手医科大学内科学第一

• 岡 達三

  徳島大学医学部栄養化学

書誌事項

タイトル別名

• Regulation of albumin gene expression in carbon tetrachloride-induced liver cell injury.

抄録

The control mechanism of albumin gene expression in carbon tetrachloride (CCl₄)-induced liver damage was investigated. Rats were injected intraperitoneally with CCl₄ (1.0ml/kg b.w.) and killed at various time intervals. The level of albumin mRNA in the liver, measured by Northern blot hybridization, was found to be 30%, 18% and 40% of the control value at 6, 12 and 24 hours after CCl₄ administration, respectively. Since the transcription rate of albumin gene, measured by a nuclear run-on assay, was decreased by 50% at 12 hours, destabilization of albumin mRNA must have also occurred in the CCl₄-poisoned liver. Gel mobility shift assay showed that DNA-binding activities of tissue-specific transcriptional factors, HNF1 and C/EBP, were decreased by 50% at 12 hours while Western blot assay indicated that the concentration of C/EBP was unchanged in CCl₄ poisoning.<BR>These results suggest that the decrease in albumin gene expression in CCl₄-induced liver injury is due to inactivation of tissue-specific transcriptional factors such as HNF1 and C/EBP and destabilization of albumin mRNA.

収録刊行物

• 肝臓

  肝臓 36 (5), 289-295, 1995

  一般社団法人　日本肝臓学会

キーワード

• CCl<SUB>4</SUB>急性肝障害
• アルブミン遺伝子発現
• 肝特異的転写調節因子

詳細情報

• CRID: 1390001204791573760
• NII論文ID: 10005570369
• NII書誌ID: AN00047770
• DOI: 10.2957/kanzo.36.289
• ISSN: 18813593
• ISSN: 04514203
• Web Site: http://www.jstage.jst.go.jp/article/kanzo1960/36/5/36_5_289/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1996005717
• 本文言語コード: ja
• データソース種別:
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  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可