書誌事項
- タイトル別名
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- Limits of nucleic acid amplification tests for diagnosis of smear negative pulmonary tuberculosis.
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抄録
Study objectives: To assess the usefulness of commercial kits of nucleic acid amplifica tion test (NAAT) for diagnosis of smear negative (SN) pulmonary tuberculosis.<BR>Design and patients: Retrospective study of patients who were diagnosed as, or sus pected of pulmonary tuberculosis during 3 years from January 1996 to December 1998 in Fukuiuii Hospital which has 100 beds for tuberculosis patients.<BR>Measurements and Results: 145 smear-negative culture-positive pulmonary tuberculosis patients are entered to our analysis. The DNA-based amplification test kit (Amplicor Mycobacterium tuberculosis Test (AMPL), Roche Diagnostic Systems, Basel, Switzerland) detected 39.2% (20/51, 95% confidence interval (CI): 25.8-52.6%) of smear-nega tive culture-positive (SNCP) pulmonary tuberculosis cases. The RNA-based amplification test kit (Gen-Probe Amplified Mycobacterium tuberculosis Direct Test (AMTDT), Gen-Probe Inc., San Diego, Calif., USA) detected 40.5% (15/37, 95% CI: 24.7-56.3%) of SNCP pulmonay tuberculosis cases. For both NAATs (AMPL and AMTDT), between two groups with and without the NAAT at diagnosis of SNCP pulmonary tuberculosis, there was statistical difference in culture-positive rate (proportion of positivity in sputum culture tests at diagnosis), but no statistical difference in maximum number of colony of Mycobacterium tuberculosis (MTB). When stratified for the culture-positive rate, adjusted sensitivity for SNCP patients was 44.2% (AMPL) and 40.4% (AMTDT) respec tively. On the other hand, among 245 patients with sputum AMPL positive results during the 3 years, 8 were smear-negative culture-negative (SNCN), only one out of these 8 cases was judged as true active tuberculosis without treatment. Among 89 patients with sputum AMTDT positive results, 7 were SNCN, and 3 out of them were judged as true active tuberculosis without treatment.<BR>Conclusion: Usefulness of commercial NAAT kits (AMPL and AMTDT) to diagnosis SN pulmonary tuberculosis is limited in the point of sensitivity.
収録刊行物
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- 結核
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結核 75 (12), 691-697, 2000
一般社団法人 日本結核病学会
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詳細情報 詳細情報について
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- CRID
- 1390282679976327168
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- NII論文ID
- 10005618197
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- NII書誌ID
- AN00073442
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- COI
- 1:STN:280:DC%2BD3M7ptF2ktw%3D%3D
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- ISSN
- 18842410
- 00229776
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- PubMed
- 11201136
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可