３層構造よりなるハイブリッド人工血管の開発：細胞の動的挙動および細胞外マトリックスの再構築

DOI 被引用文献1件 参考文献6件

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タイトル別名
  • Reconstruction of a Hybrid Vascular Graft with Three cell types.

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We developed hybrid grafts hierarchically incorporated with endothelial cells (ECs), smooth muscle cells (SMCs) and fibroblasts (FCs), and investigated the cellular behaviors and ultrastructure of regenerated extracellular matrix (ECM) of those grafts in vivo. Two models of hybrid vascular grafts were constructed on knitted Dacron grafts (ID=4mm, Length; 6cm) in vitro. Model I grafts were hierarchically structured with a confluent monolayer of ECs, a middle layer of SMCs and an outer layer of FCs. Model II grafts consisted of a confluent EC monolayer and a homogeneously mixed layer composed of SMCs and FCs pre-labelled with a fluorescent lipid. The designed artificial ECM was a mixed gel of type I collagen and dermatan sulfate. Both grafts were implanted bilaterally in canine carotid arteries of 14 dogs for up to 12 weeks without anticoagulant. Regardless of model type, patent grafts (n=24 out of 28 implanted grafts) were completely endothelialized. In model II grafts at 4 weeks, SMCs migrated to the subendothelial layer, whereas labelled FCs migrated to an outer layer, which was identified under a fluorescent microscope. Observation of both grafts at 12 weeks showed that most of the collagen fibers in the luminal surface layer were longitudinally oriented, but those in deeper layers tended to orient circumferentially, and that newly generated elastic tissues were composed of many concentric laminae. SMCs of both grafts at 12 weeks were predominantly of the contractile phenotype. Thus, it can be said that hybrid grafts incorporating intima-, media- and adventitia-type cell layers can significantly enhance the process of arterial wall reconstruction.

収録刊行物

  • 人工臓器

    人工臓器 24 (1), 150-155, 1995

    一般社団法人 日本人工臓器学会

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