Rat Gastric Mucins Recognized by Monoclonal Antibodies RGM21 and HIK1083: Isolation of Mucin Species Characteristic of the Surface and Glandular Mucosa.

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  • Goso Yukinobu
    Department of Biochemistry, Kitasato University School of Medicine
  • Ishihara Kazuhiko
    Department of Biochemistry, Kitasato University School of Alied Health Science
  • Kurihara Makoto
    Isehara Research laboratory, Kanto Chemical Co., Inc
  • Sugaya Tsukiko
    Department of Biochemistry, Kitasato University School of Medicine
  • Hotta Kyoko
    Department of Biochemistry, Kitasato University School of Medicine

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Whole mucins and reduced subunits were extracted from the corpus of the rat stomach. After purification by Sepharose CL-4 B chromatography followed by cesium trifluoroace-tate equilibrium centrifugation, they were analyzed by Sepharose CL-2 B chromatography, rate-zonal sedimentation centrifugation, and Q-Sepharose chromatography. Monoclonal antibodies RGM 21 and HIK 1083, which histochemically stained mucins in the surface and glandular mucosa of the rat stomach, respectively, were used to detect the site-specific mucins. Although RGM 21- and HIK 1083-reactive mucins both had a multimerized structure, the density and size of both the whole mucins and reduced subunits differed, thus indicating the presence of distinct mucin species in the surface and glandular mucosa. The mucin subunits were separated into four fractions, UB, B 1, B2a, and B2b, by Q-Sepharose chromatography. HIK 1083 reacted mainly with UB, while RGM 21 reacted with B 1, B2a, and B2b. These results, combined with dot-blot, amino acid, and carbohydrate composition analyses, showed that the surface mucins may consist of three kinds of subunits. In contrast, the glandular mucins may consist of one kind of subunit which differs from that of surface mucins.

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