書誌事項
- タイトル別名
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- Colorimetric bioassay for measuring aminoglycoside antibiotics in blood spots on filter paper. Individual application to netilmicin, gentamicin and isepamicin.
- Individual application to netilmicin, gentamicin and isepamicin
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抄録
A simplified microbiological detection method for aminoglycoside antibiotics, using Bacillus subtilis and thymolphthalein as calorimetric indicator, is described. In each test, netilmicin, gentamicin and isepamicin were sufficiently recovered from individual blood spots during incubation in the same medium. After incubation, the concentration of blue-colored thymolphthalein was determined by both fluorescence polarization immunoassay and a spectrophotometer equipped with a multichannel photodiode array detector for each aminoglycoside. There was proportionality between the concentration of each aminoglycoside and the degree of discoloration of thymolphthalein. No difference was found between these two methods. The advantage of this method is that the approximate individual concentration ofnetilmicin, gentamicin or isepamicin can also be detected visually by using a paper disc containing thymolphthalein for the individual aminoglycoside as follows: navy blue, more than peak concentration; light blue, within therapeutic range; white, less than trough concentration, and ready for injection.
収録刊行物
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- 日本化学療法学会雑誌
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日本化学療法学会雑誌 43 (11), 1041-1047, 1995
公益社団法人 日本化学療法学会
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詳細情報 詳細情報について
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- CRID
- 1390282681261958912
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- NII論文ID
- 10005862745
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- NII書誌ID
- AN10472127
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- ISSN
- 18845886
- 13407007
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可