書誌事項
- タイトル別名
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- Effect of DNA Topoisomerase I Inhibitor on the Transduction Efficiency of an deno-associated Virus Vector in Human Airway Epithelial Cells.
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抄録
We tested the effects of a DNA topoisomerase inhibitor (camptothecin; CPT) on the transduction efficiency of AAV vectors in cultured human airway epithelial cells. The cells were treated with CPT for 24 hours, then exposed to AAV-CMV-LacZ for 1 hour at different multiplicities of infection (moi). Transduction efficiency of AAV vectors was assessed using X-gal staining as the percentage of LacZexpressing cells. The transduction efficiency was approximately 1.5 to 10 fold increased by treatment with CPT prior to AAV vector exposure. However, treatment with CPT after AAV vector infection did not enhance the transduction efficiency of the vectors. These results suggest that pre-treatment with CPT increases the transduction efficiency of AAV vectors, probably by nodulating cellular function.
収録刊行物
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- 日本胸部疾患学会雑誌
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日本胸部疾患学会雑誌 35 (12), 1312-1317, 1997
社団法人 日本呼吸器学会
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詳細情報 詳細情報について
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- CRID
- 1390282679995342720
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- NII論文ID
- 130003679430
- 10006191765
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- NII書誌ID
- AN00187758
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- ISSN
- 1883471X
- 03011542
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- PubMed
- 9567074
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可