Identification of Colloidal Gold Labels with Template-Matching Method for Atomic Force Microscopy

• Okamoto Hiroshi

  Graduate School of Engineering, Toyo University

• Ohta Yuji

  Department of Mechanical Engineering, Faculty of Engineering, Toyo University

• Okuda Tomoko

  Department of Mechanical Engineering, Faculty of Engineering, Toyo University

抄録

Atomic Force Microscopy has become a powerful tool in the fields of biophysics and biophysical chemistry. A number of biological samples have been imaged at high resolution by AFM. One of the problems of imaging biological samples with AFM is the lack of specificity, and the need for a specific labeling technique for AFM has been recognized. Immuno-gold labels in combination with AFM have been developed for that purpose. However, the colloidal gold labels can hardly be discriminated from the globular protrusions of the cell membrane.<br>  In this paper, a numerical template-matching method was developed to discriminate the colloidal gold label from the globular protrusion. By applying this method to sheep erythrocytes labeled with 10 nm colloidal gold conjugated antibody on the surface, the position of the colloidal gold label corresponding to the antigen could be determined. This method will be useful for discrimination of a cell surface antigen by AFM.

収録刊行物

• bioimages

  bioimages 8 (4), 121-127, 2000

  日本バイオイメージング学会

キーワード

• atomic force microscopy
• immuno-gold labeling
• pattern analysis
• template-matching

詳細情報

• CRID: 1390285300160604416
• NII論文ID: 10006232143
• NII書誌ID: AA11084187
• DOI: 10.11169/bioimages.8.121
• ISSN: 09192719
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可