Construction of a BAC library derived from the inbred Hd-rR strain of the teleost fish, Oryzias latipes.
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- Matsuda Masaru
- Laboratory of Reproductive Biology, National Institute for Basic Biology
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- Kawato Norihisa
- Graduate School of Science, Nagoya University
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- Asakawa Shuichi
- Department of Molecular Biology, Keio University School of Medicine
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- Shimizu Nobuyoshi
- Department of Molecular Biology, Keio University School of Medicine
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- Nagahama Yoshitaka
- Laboratory of Reproductive Biology, National Institute for Basic Biology
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- Hamaguchi Satoshi
- Department of Environmental Science, Faculty of Science, Niigata University
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- Sakaizumi Mitsuru
- Department of Environmental Science, Faculty of Science, Niigata University
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- Hori Hiroshi
- Graduate School of Science, Nagoya University
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Abstract
A large insert genomic bacterial artificial chromosome (BAC) library was constructed from the inbred Hd-rR strain of the medaka, Oryzias latipes. Approximately 92, 000 clones were gridded on high-density replica filters. Insert analysis of randomly selected clones indicated a mean insert size of 210 kb and predicted a 24 times coverage of the medaka genome. The library was hybridized with a single locus DNA fragment, and the resulting positive clones were characterized and shown to be compatible with a 24-fold redundant library. This first large insert genomic library of the medaka should increase the speed of genomic analyses for this fish species.
Journal
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- Genes & Genetic Systems
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Genes & Genetic Systems 76 (1), 61-63, 2001
The Genetics Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282680422607744
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- NII Article ID
- 10006698255
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- NII Book ID
- AA11077421
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- COI
- 1:CAS:528:DC%2BD3MXktVaktrc%3D
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- ISSN
- 18805779
- 13417568
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- NDL BIB ID
- 5716836
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- PubMed
- 11376553
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed
