Detection and Localization of a Ca〔2+〕-ATPase Activity in Toxoplasma gondii

• Bouchot André

  UPRES EA 2070 et Laboratoire de Parasitologie-Mycologie

• Jaillet Jean-David

  UPRES EA 2070 et Laboratoire de Parasitologie-Mycologie

• Bonhomme Annie

  UPRES EA 2070 et Laboratoire de Parasitologie-Mycologie

• Alessandro Nathalie Pezzella-D'

  UPRES EA 2070 et Laboratoire de Parasitologie-Mycologie

• Laquerriere Patrice

  Laboratoire de Microscopie Electronique Analytique et Quantitative, IFR 53

• Kilian Laurence

  Laboratoire de Microscopie Electronique Analytique et Quantitative, IFR 53

• Burlet Henriette

  UPRES EA 2070 et Laboratoire de Parasitologie-Mycologie

• Gomez-Marin Jorge-Enrique

  Groupo de Pathologia Infecciosa, Departmento de Medicina Interna, Hospital San Juan de Dios, Universidad Nacional de Colombia

• Pluot Michel

  Laboratoire d'Anatomie-Pathologique, Hôpital Robert Debré

• Bonhomme Pierre

  Laboratoire de Microscopie Electronique Analytique et Quantitative, IFR 53

• Pinon Jean-Michel

  UPRES EA 2070 et Laboratoire de Parasitologie-Mycologie

書誌事項

タイトル別名

• Detection and Localization of a Ca2+-ATPase Activity in Toxoplasma gondii.

抄録

Toxoplasma gondii, the agent causing toxoplasmosis, is an obligate intracellular protozoan parasite. A calcium signal appears to be essential for intracellular transduction during the active process of host cell invasion. We have looked for a Ca²⁺-transport ATPase in tachyzoites and found Ca²⁺-ATPase activity (11-22 nmol Pi liberated/mg protein/min) in the tachyzoite membrane fraction. This ATP-dependent activity was stimulated by Ca²⁺ and Mg²⁺ ions and by calmodulin, and was inhibited by pump inhibitors (sodium orthovanadate or thapsigargin). We used cytochemistry and X-ray microanalysis of cerium phosphate precipitates and immunolabelling to find the Ca²⁺, Mg²⁺-ATPase. It was located mainly in the membrane complex, the conoid, nucleus, secretory organelles (rhoptries, dense granules) and in vesicles with a high calcium concentration. Thus, Toxoplasma gondii possesses Ca²⁺-pump ATPase (Ca²⁺, Mg²⁺-ATPase) as do eukaryotic cells.

収録刊行物

• Cell Structure and Function

  Cell Structure and Function 26 (1), 49-60, 2001

  日本細胞生物学会

キーワード

• Ca²⁺
• Mg²⁺
• ATPase
• <i>Toxoplasma gondii</i>
• cerium precipitation
• X-ray microanalysis
• immunolocalization

詳細情報

• CRID: 1390282679673567360
• NII論文ID: 130004137384
• NII論文ID: 10006904686
• NII書誌ID: AA0060007X
• DOI: 10.1247/csf.26.49
• COI: 1:CAS:528:DC%2BD3MXjvVyjurg%3D
• ISSN: 13473700
• ISSN: 03867196
• NDL書誌ID: 5738699
• PubMed: 11345503
• Web Site: http://id.ndl.go.jp/bib/5738699
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5738699
• Web Site: http://www.jstage.jst.go.jp/article/csf/26/1/26_1_49/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2002072957
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可