The Mechanism of Maitotoxin-Induced Elevation of the Cytosolic Free Calcium Level in Rat Cerebrocortical Synaptosomes.

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  • Satoh Eiki
    Department of Pharmacology, University of Obihiro School of Veterinary Medicine
  • Ishii Toshiaki
    Department of Pharmacology, University of Obihiro School of Veterinary Medicine
  • Nishimura Masakazu
    Department of Pharmacology, University of Obihiro School of Veterinary Medicine

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  • Mechanism of Maitotoxin Induced Elevation of the Cytosolic Free Calcium Level in Rat Cerebrocortical Synaptosomes

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Abstract

The present study was conducted to elucidate the mechanism of the maitotoxin (MTX)-induced increase in intrasynaptosomal free calcium level ([Ca2+]i). The MTX (1 ng/ml)-induced increase in [Ca2+]i was partially inhibited by the omission of extracellular Ca2+ (Ca2+e) or the addition of verapamil, but not by adding nifedipine, ω-agatoxin IVA, ω-conotoxin GVIA and ω-conotoxin MVIIC. An increase in [Ca2+]i in the absence of Ca2+e was sensitive to procaine, TMB-8, genistein and verapamil, but not to ryanodine and U-73122. These results may suggest that MTX increases [Ca2+]i by stimulating Ca2+ entry through voltage-independent nonselective cation channels and Ca2+ release from stores through a phospholipase C-γ1-mediated pathway in rat cerebrocortical synaptosomes.

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