ビタミンK3添加化学発光法を用いた細胞致死性の測定

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タイトル別名
  • Chemiluminescent Cytotoxicity Test for Food Stuff with Vitamin K3.
  • ビタミン K3 テンカ カガク ハッコウホウ オ モチイタ サイボウ チシセイ

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抄録

The cytotoxicity of foods was determined on the base of the rate of vitamin K3-catalyzed H202production by mammalian cells. Vitamin K3-catalyzed H202 production and the chemiluminescent assay of H202 require 10min and 5 s, respectively. This cytotoxicity test was useful for the rapid detection of weak cytotoxic antioxidants such as BHA and BHT, and the preservatives such as esters of p-hydroxybenzoate. These cytotoxic effects were observed a few hours after the incubation with NIH/3 T 3 cells. The cytotoxic effects of alcoholic drinks, non-alcoholic drinks and liquid foods were also observed 24 h after the addition of drinks or foods to NIH/3 T 3 cells.

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