人工レクチン能を有するボロン酸基含有ポリマーへのアミノ基の導入によるリンパ球増殖活性の増強

• 内村 英一郎

  東京理科大学 基礎工学部材料工学科

• 宮崎 浩明

  東京理科大学 基礎工学部材料工学科

• 片岡 一則

  東京理科大学 基礎工学部材料工学科

• 岡野 光夫

  東京女子医科大学 医用工学施設

• 桜井 靖久

  東京女子医科大学 医用工学施設

書誌事項

タイトル別名

• Synthesis of artificial lectin-polymer, having mitogenic function for lymphocytes.

抄録

We have found that water soluble polymer having phenylboronic acid moiety has ability to induce mouse lymphocytes proliferation. This might be due to the binding of phenylboronic acid residues m e polymer to sugar residues existing on the plasma membrane surface of lymphocytes. In this context, phenylboronate polymer serves as artificial mitogen of lymphocytes. Phenylboronic acid is known to have an equilibrium between trigonal form and tetrahedral rate form in the aqueous milieu. Only tetrahedral borate form a stable complex with sugar. However due to the relatively high pKa of phenylboronic acid, there exists only a small amount of tetrahedral boronate at physiological condition (pH7.4). To increase mitogemc activity under physiological condition, amino groups were introduced into the borate-contaming polymer in this study to facilitate complex formation of boronate with sugar residues on the plasma membrane, allowing to achieve an increased ability to proliferate lymphocytes Thus, ternary copolymer (DBA) of N, N-dimethylacrylamide, 3-acrylamidephenylboronic acid and N, N-dimethylaminopropylacrylamide (DMAPAA) was synthesized. Proliferative response of mouse lymphocytes was evaluated from incorporation of ³H-thymidine. Remarkable increase in ³-thymidine uptake to lymphocytes was observed for lymphocytes cultured with DBA compared to that with DB which has no amino groups. This increase in mitogenic ability seems to be due to the effective formation of the complex between boronate and sugar residues on the cell membrane because of the cooniination of amino groups to phenylboronic acid moieties in DBA. There was observed an optnnum amino group content in the DBA copolymer in terms of lymphocyte proliferation, indicating a crucial role of amino group content of DBA in lymphocyte proliferation.

収録刊行物

• 人工臓器

  人工臓器 26 (2), 472-475, 1997

  一般社団法人 日本人工臓器学会

キーワード

• Lymphocytes proliferation
• Mitogen
• Artificial Lectin
• Phenylboronic acid
• amino group

詳細情報

• CRID: 1390282679932934912
• NII論文ID: 10007718720
• NII書誌ID: AN00120167
• DOI: 10.11392/jsao1972.26.472
• ISSN: 18836097
• ISSN: 03000818
• Web Site: https://search.jamas.or.jp/link/ui/1997165794
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可