製膜条件により分子ふるい効果を制御したコラーゲンゲル膜の作製

• 土生 拓史

  物質工学工業技術研究所

• 酒井 清孝

  早稲田大学理工学部化学工学

• 金森 敏幸

  早稲田大学理工学部化学工学

書誌事項

タイトル別名

• Preparation of collagen-gel flat membranes with various microstructures and solute permeabilities.

抄録

We tried to control mass transport and microstructure in collagen gel applied to cell culture medium. An aqueous solution of type I atelocollagen of pigskin was cast into a glass Petn-dish and dried to control collagen concentration. Then, the semi-dried film of collagen was crosslnked with glutaraldehyde aqueous solution saturated by sodium chloride. Finally, a collagen-gel flat membrane was obtained after washing the crosslinked thin film with excess pure water. Analysis of intramembrane diffusivity of the membranes using the tortuous pore diffusion model represented that glutaraldehyde concentration at crosslinkng had no effect on microstructure of the membranes. On the other hand, intramembrane diffusivity of the membranes was depend on collagen concentration at crosslinking in the range of 77-96 wt%. The collagen concentration was also correlated with structural parameters of the membranes. In conclusion, we could obtain various collagen-gel flat membranes with different solute permeabilities and microstructures.

収録刊行物

• 人工臓器

  人工臓器 27 (1), 244-249, 1998

  一般社団法人 日本人工臓器学会

キーワード

• collagen gel
• solute permeability
• tortuous pore diffusion model

詳細情報

• CRID: 1390001204955859712
• NII論文ID: 10007719924
• NII書誌ID: AN00120167
• DOI: 10.11392/jsao1972.27.244
• ISSN: 18836097
• ISSN: 03000818
• Web Site: https://search.jamas.or.jp/link/ui/1998162939
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可