新たに発見された有機水銀分解遺伝子merB3とその発現調節系を利用した有機水銀の検出方法に関する研究 Study on the Organomercury Detection Method Using a New Organomercury Lyase Gene, merB3, and a Regulation System of the Gene Expression
Mercurials have been released into the environment by geological or anthropogenic activities. Organomercurial compounds such as methylmercury are known as toxic compounds. Therefore, organomercurials should be monitored to keep clean environments. However, chemical species of organomercurials are not easily detected separately or individually.<br>In this study, we tried to detect organomercurial compounds by using a bacterial gene-expression system. A series of <i>mer</i> operon-luciferase (<i>mer-lux</i>) transcriptional fusion plasmids (pHYΔB3Lux and pHYB3Lux) was constructed to evaluate the gene expression system with a new organomercury lyase gene <i>merB3</i> from <i>Bacillus megaterium</i> MB1. A plasmid (pGR1A) encoding <i>mer</i> operon genes from O/P<sub>merR1</sub> to <i>merA</i> isolated from <i>B. megaterium</i> MB1 was used as a transacting gene expression vector with the <i>mer-lux</i> transcriptional fusion plasmids into a same host bacterial cell. The transformants that carry a set of two plasmids (pHYΔB3Lux+pGR1A or pHYB3Lux+pGR1A), respectively) were used for detection of organomercurials. The experimental results showed that the transformant with pHYΔB3Lux+pGR1A responded to only mercury chloride (MC). On the other hand, the transformant with pHYB3Lux+pGR1A responded and detected MC and the all organomercurials tested in the study. Therefore, the bacterial strain which possess a gene expression system of <i>mer-lux</i> transcriptional fusion was useful to detect organomercurials with distinction from inorganic mercury.
- 水環境学会誌 = Journal of Japan Society on Water Environment
水環境学会誌 = Journal of Japan Society on Water Environment 24(4), 219-224, 2001-04-10
Japan Society on Water Environment