PCR-DGGEによる津久井湖における藻類を含む微生物群集構造の季節変動解析 Analysis of Seasonal and Structural Changes in Microbial Community including Algae in Tsukui Lake by PCR-DGGE

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Seasonal change of microbial community including bacteria and algae in Tsukui Lake, an eutrophic reservoir in Kanagawa prefecture, was investigated by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR) amplified fragments of 16S rDNA from May to October in 2000. A sudden algal bloom of cyanobacteria (<i>Microcystis</i> sp. and <i>Anabaena</i> sp.) after a heavy storm in July was observed both from a traditional algal cell counting and from a DGGE banding pattern analysis. In order to express graphically the seasonal change of the community based on the dissimilarity index, multi-dimensional scaling (MDS) was applied. The MDS map revealed a cyclic change of the community and a distinctive divergence from the path during the agal bloom. Since the divergence disappeared in an analysis with the DGGE image excluding the bands of cyanobacteria, the major part of the community appeared in the DGGE image might be independent of the sudden algal bloom. Dissimilarity of microbial communities was much lower among three different sampling sites than among the sampling months.

収録刊行物

  • 水環境学会誌 = Journal of Japan Society on Water Environment

    水環境学会誌 = Journal of Japan Society on Water Environment 24(12), 856-864, 2001-12-10

    Japan Society on Water Environment

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各種コード

  • NII論文ID(NAID)
    10008006196
  • NII書誌ID(NCID)
    AN10372439
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09168958
  • NDL 記事登録ID
    6007386
  • NDL 雑誌分類
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL 請求記号
    Z16-1045
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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