Analysis of Seasonal and Structural Changes in Microbial Community including Algae in Tsukui Lake by PCR-DGGE.

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  • KASUGA Ikuro
    Graduate School of Engineering, University of Tokyo
  • FURUMAI Hiroaki
    Graduate School of Engineering, University of Tokyo
  • NAKAJIMA Fumiyuki
    Graduate School of Engineering, University of Tokyo
  • ARII Suzue
    Tanigahara Water Purification Plant, Water Works Bureau, Public Enterprises Agency, Kanagawa Prefecture

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Other Title
  • ＰＣＲ‐ＤＧＧＥによる津久井湖における藻類を含む微生物群集構造の季節変動解析
  • PCR-DGGEによる津久井湖における藻類を含む微生物群集構造の季節変動解析
  • PCR DGGE ニ ヨル ツクイコ ニ オケル ソウルイ オ フクム ビセイブツ グンシュウ コウゾウ ノ キセツ ヘンドウ カイセキ

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Abstract

Seasonal change of microbial community including bacteria and algae in Tsukui Lake, an eutrophic reservoir in Kanagawa prefecture, was investigated by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR) amplified fragments of 16S rDNA from May to October in 2000. A sudden algal bloom of cyanobacteria (Microcystis sp. and Anabaena sp.) after a heavy storm in July was observed both from a traditional algal cell counting and from a DGGE banding pattern analysis. In order to express graphically the seasonal change of the community based on the dissimilarity index, multi-dimensional scaling (MDS) was applied. The MDS map revealed a cyclic change of the community and a distinctive divergence from the path during the agal bloom. Since the divergence disappeared in an analysis with the DGGE image excluding the bands of cyanobacteria, the major part of the community appeared in the DGGE image might be independent of the sudden algal bloom. Dissimilarity of microbial communities was much lower among three different sampling sites than among the sampling months.

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