Fructose Biosensor Based on D-fructose Dehydrogenase and Phenanthroline Cobalt Complex as a Mediator

• WATANABE Shinya

  Department of Bioengineering, Faculty of Engineering, Soka University

• KUBO Izumi

  Department of Bioengineering, Faculty of Engineering, Soka University

Abstract

Fructose biosensor based on D-Fructose dehydrogenase (FDH) was developed with use of tris (1,10-phenanthroline) cobalt (II) complex (Co(phen)₃²⁺) as a redox mediator. Co(phen)₃²⁺ could be prepared easily and was oxidized reversibly at low potential. For the immobilization of FDH, a gold electrode was modified through chemisorption of cystamine. FDH was covalently bound to self-assembled monolayer of cystamine by cross-linking amino groups of cystamine and FDH molecule using glutaraldehyde. With use of Co(phen)₃²⁺ as a redox mediator, the catalytic current of the FDH-modified gold electrode increased by the addition of D-fructose. In the immobilization procedure, 0.2 mg/ml of FDH resulted in the largest catalytic response among other concentration of FDH. The best sensitivity of the sensor was obtained at pH 7.0 and 35°C. The calibration curve exhibits a linear relationship between 10 and 110 µM with a correlation coefficient of 0.971 and the relative standard deviation of 0.57% (n = 3).

Journal

• Electrochemistry

  Electrochemistry 70 (4), 258-263, 2002-04-05

  The Electrochemical Society of Japan

Keywords

• Fructose Sensor
• Fructose Dehydrogenase
• Tris(l,10-phenanthroline) Cobalt
• Gold

Details

• CRID: 1390564227301260288
• NII Article ID: 10008009506
• NII Book ID: AN00151637
• DOI: 10.5796/electrochemistry.70.258
• ISSN: 21862451
• ISSN: 13443542
• NDL BIB ID: 6128144
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6128144
• Web Site: https://www.jstage.jst.go.jp/article/electrochemistry/70/4/70_258/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Allowed