神経-グリア細胞系におけるカルシウム波とカルシウム振動 : その測定と発生機構 Observation of Calcium Activities in Newron-Glia Co-cultured System : Seacrhing for underling mechanism

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Cultured neural network is a useful tool for the understanding of fundamental functions and structure of nervous system. In this biological neural network, nerve cells mutually communicate by electrical or chemical interactions, and exhibit collective dynamics of activities such as synchronous bursting or calcium wave propagation. Photostimulation by using caged-glutamate were performed to elucidate the mechanism of Ca wave propagations through the measurement of the speed of Ca wave, rising time of Ca increase, and range of propagation, etc. To analyze the activity and dynamical connectivity of the cultured network, we quantified spontaneous collective dynamics of the network by lowering magnesium concentration in extracellular solution. From the visualization of network structure, cell type discrimination by MAP2 monochronal antibody, and measurement of dynamical activity by [Ca<SUP>2+</SUP>] sensitive dye, we quantitatively elucidated that collectivity of the network activities were increased due to the development of long-range interaction between neurons.

収録刊行物

  • 膜 26(6), 251-258, 2001-11-01

    THE MEMBRANE SOCIETY OF JAPAN

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各種コード

  • NII論文ID(NAID)
    10008010876
  • NII書誌ID(NCID)
    AN0023215X
  • 本文言語コード
    JPN
  • 資料種別
    REV
  • ISSN
    03851036
  • NDL 記事登録ID
    5992376
  • NDL 雑誌分類
    ZR2(科学技術--生物学--生化学)
  • NDL 請求記号
    Z18-1127
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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