牛卵母細胞体外成熟での透明帯遺伝子発現に及ぼすFSHおよびLHの影響

  • 渡辺 伸也
    農水省九州農業試験場 Present: Agriculture, Forestry and Fisheries Research Council Secretariat, Ministry of Agriculture, Forestry and Fisheries
  • 中西 裕二
    農水省九州農業試験場

書誌事項

タイトル別名
  • Effect of Follicle-stimulating Hormone and Luteinizing Hormone on Zona Pellucida Gene Expression during Bovine Oocyte Maturation In Vitro.

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抄録

Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in bovine oocyte maturation. This study tested the effect of FSH and LH on bovine ZP gene expression during oocyte maturation in vitro with RNase protection assay. These gonadotropins had no effect on ZP gene expression in bovine oocytes cultured for 5 or 15 hours, but significant suppression of germinal vesicle breakdown (GVB) and metaphase II (MII) generation were observed with FSH. These data suggest that neither FSH nor LH has a significant effect on ZP gene expression in bovine oocytes. And the ZP gene expression could be independent of the mechanism of oocyte nuclear maturation in cattle.

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