胚性幹 (ES) 細胞に導入されたpMGN(-4k)LacZ-neo遺伝子のキメラマウス胎子における発現

• 小薗井 真人

  岩手大学農学部動物科学講座 Present address: Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University

• 伊藤 和衛

  岩手大学農学部動物科学講座

• 高橋 寿太郎

  岩手大学農学部動物科学講座

書誌事項

タイトル別名

• Expression of pMGN(-4k)LacZ-neo Gene Introduced into Embryonic Stem (ES) Cells in Chimeric Mouse Fetuses.

抄録

We attempted to produce chimeric mouse fetuses by using an embryonic stem (ES) cell subline, OM 3, which were transfected with pMGN(-4k)LacZ-neo transgene, and to analyze expression of the transgene in chimeric mouse fetuses. In embryonic days 11-13 (E11-13), no fetuses expressing the transgene were observed. In E14, three of 11 normal fetuses recovered showed the transgene expression. In these chimeric mouse fetuses, the regions expressing the transgene were found in a wide range of fetuses, including the temporal region of the head, trunk from neck to tail, and limbs. It was shown by histochemical analysis that these patterns of transgene expression were specific to skeletal muscles. These results indicated that OM 3 cells might be useful for analyses of mouse skeletal-myogenesis and for the development of basic techniques for skeletal muscles in regenerative medicine.

収録刊行物

• 日本哺乳動物卵子学会誌

  日本哺乳動物卵子学会誌 18 (3), 123-126, 2001

  日本卵子学会

キーワード

• 胚性幹(ES)細胞
• マイオジェニン遺伝子発現
• LacZレポーター遺伝子
• キメラマウス胎子
• 骨格筋形成

詳細情報

• CRID: 1390282679315370368
• NII論文ID: 10008024570
• NII書誌ID: AN10548943
• DOI: 10.1274/jmor.18.123
• ISSN: 13475878
• ISSN: 13417738
• NDL書誌ID: 5982111
• Web Site: https://iwate-u.repo.nii.ac.jp/records/9157
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030640758
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5982111
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可