代謝プロファイルテストのためのウシ血液の採取・保存方法の検討

• 岡田 啓司

  岩手大学農学部附属家畜病院

• 安田 準

  岩手大学農学部附属家畜病院

書誌事項

タイトル別名

• Methods for Collection and Preservation of Cattle Blood for Metabolic Profile Test
• タイシャ プロファイル テスト ノ タメ ノ ウシ ケツエキ ノ サイシュ ホゾン ホウホウ ノ ケントウ

抄録

Conditions for collecting and preserving blood samples for the metabolic profile test (MPT) were investigated by using five healthy adult castrated Japanese Black cattle. Values in heparinated plasma were compared with those in rapidly separated serum : free fatty acid (FFA), β hydroxy-butyric acid (BHB), total cholesterol (T-cho), phospholipids (PL), aspartate aminotransferase (AST) and γ glutamyl transpeptidase (γ-GTP) were lower in plasma, while β lipoprotein (β-lipo), total protein (Tp) and albumin (Alb) were higher in plasma. When whole blood was stored at room temperature for 2 hours, urea nitrogen (BUN) and γ-GTP were elevated in serum. Regarding glucose (Glu) level there was no difference between serum and heparinated plasma. When separated serum was refrigerated or frozen, Glu, BHB, T-cho, Tp, Alb, BUN and inorganic phosphor were stable for 7 days. FFA, PL, β-Lipo, Ca, AST and γ-GTP were relatively unstable.

収録刊行物

• 日本家畜臨床学会誌

  日本家畜臨床学会誌 24 (1), 13-18, 2001

  日本家畜臨床学会 ・ 大動物臨床研究会

キーワード

• 採血
• 代謝プロファイルテスト
• 血漿
• 血清
• 保存

詳細情報

• CRID: 1390001205225811840
• NII論文ID: 10008024620
• NII書誌ID: AA1163152X
• DOI: 10.4190/jjvc2001.24.13
• ISSN: 18834604
• ISSN: 13468464
• NDL書誌ID: 5848543
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030631485
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5848543
• Web Site: https://search.jamas.or.jp/link/ui/2002057174
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可