Methods for Collection and Preservation of Cattle Blood for Metabolic Profile Test

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  • Okada K.
    Veterinary Teaching Hospital, Faculty of Agriculture, Iwate University
  • Yasuda J.
    Veterinary Teaching Hospital, Faculty of Agriculture, Iwate University

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  • 代謝プロファイルテストのためのウシ血液の採取・保存方法の検討
  • タイシャ プロファイル テスト ノ タメ ノ ウシ ケツエキ ノ サイシュ ホゾン ホウホウ ノ ケントウ

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Abstract

Conditions for collecting and preserving blood samples for the metabolic profile test (MPT) were investigated by using five healthy adult castrated Japanese Black cattle. Values in heparinated plasma were compared with those in rapidly separated serum : free fatty acid (FFA), β hydroxy-butyric acid (BHB), total cholesterol (T-cho), phospholipids (PL), aspartate aminotransferase (AST) and γ glutamyl transpeptidase (γ-GTP) were lower in plasma, while β lipoprotein (β-lipo), total protein (Tp) and albumin (Alb) were higher in plasma. When whole blood was stored at room temperature for 2 hours, urea nitrogen (BUN) and γ-GTP were elevated in serum. Regarding glucose (Glu) level there was no difference between serum and heparinated plasma. When separated serum was refrigerated or frozen, Glu, BHB, T-cho, Tp, Alb, BUN and inorganic phosphor were stable for 7 days. FFA, PL, β-Lipo, Ca, AST and γ-GTP were relatively unstable.

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