Purinoceptor-Mediated Calcium Mobilization and Cellular Proliferation in Cultured Bovine Corneal Endothelial Cells.

• Ho Cha Seok

  Department of Pharmacology and Toxicology,Kyorin University School of Medicine,Mitaka,Tokyo 181-8611,Japan

• Hahn Tae-Won

  Department of Ophthalmology,Catholic University Medical College,Kangnam St.Mary’s Hospital,505 Banpo-dong,Seocho-gu,Seoul 137-701,Korea

• Sekine Takashi

  Department of Pharmacology and Toxicology,Kyorin University School of Medicine,Mitaka,Tokyo 181-8611,Japan

• Lee Kweon-Haeng

  Department of Pharmacology,Catholic University Medical College,505 Banpo-dong,Seoul 137-701,Korea

• Endou Hitoshi

  Department of Pharmacology and Toxicology,Kyorin University School of Medicine,Mitaka,Tokyo 181-8611,Japan

Abstract

In the present study, we investigated the effect of adenosine triphosphate(ATP)on cytosolic free calcium mobilization and mitogenic activity in cultured bovine corneal endothelial cells(BCEC).The[Ca²⁺]_i was determined using a Ca²⁺ sensitive indicator, Fura-2/AM, and cell proliferation was evaluated by counting the cell number.ATP, its metabolites and analogs caused transient increase in[Ca²⁺]_i in a concentration-dependent manner(10^-7M−10^-3M)and the potency of agonists was ordered as follows:2-methylthio-ATP>uridine triphosphate>ATP>adenosine diphosphate.Adenosine monophosphate and adenosine did not affect[Ca²⁺]_i.ATP(10^-4M)also promoted the accumulation of inositol trisphosphate(IP₃).The ATP-induced transient[Ca²⁺]_i increase and IP₃ accumulation were attenuated by pretreatment with a phospholipase C inhibitor, U-73122(5μM), for 30 min.ATP(10^-5M)significantly enhanced the proliferation of BCEC.ATP-induced[Ca²⁺]_i increase and cell proliferation were inhibited by a purinoceptor antagonist, suramin(10^-4M).Thus, the present study indicates that BCEC contain P2 purinoceptors that regulate their proliferation.

Journal

• The Japanese Journal of Pharmacology

  The Japanese Journal of Pharmacology 82 (3), 181-187, 2000

  The Japanese Pharmacological Society

Keywords

• Corneal endothelium
• Adenosine triphosphate
• Cytosolic free calcium
• Proliferation

Details

• CRID: 1390001204284874752
• NII Article ID: 10008183354
• NII Book ID: AA00691188
• DOI: 10.1254/jjp.82.181
• COI: 1:CAS:528:DC%2BD3cXitFOitbs%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL BIB ID: 5320972
• PubMed: 10887948
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5320972
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819306833?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819306833?httpAccept=text/plain
• Web Site: https://www.jstage.jst.go.jp/article/jjp/82/3/82_3_181/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2000165065
• Text Lang: en
• Data Source:
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  • NDL
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  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed